UBA3 polyclonal antibody
产品名称： UBA3 polyclonal antibody
英文名称： UBA3 polyclonal antibody
产品编号： PAB1775
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of UBA3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human UBA3.
- Host:
- Rabbit
- Reactivity:
- Human, Mouse
- Form:
- Liquid
- Purification:
- Protein G purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8.
Walden H, Podgorski MS, Schulman BA.Nature. 2003 Mar 20;422(6929):330-4.
- 2.
- A new NEDD8-ligating system for cullin-4A.
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S.Genes Dev. 1998 Aug 1;12(15):2263-8.
- Applications
- Western Blot (Tissue lysate)
- Western blot analysis of mouse heart tissue lysate (35 ug/lane) with UBA3 polyclonal antibody (Cat # PAB1775).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with UBA3 polyclonal antibody (Cat # PAB1775) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Entrez GeneID:
- 9039
- Protein Accession#:
- Q8TBC4
- Gene Name:
- UBA3
- Gene Alias:
- DKFZp566J164,MGC22384,UBE1C,hUBA3
- Gene Description:
- ubiquitin-like modifier activating enzyme 3
- Omim ID:
- 603172
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq
- Other Designations:
- Nedd8-activating enzyme hUba3,UBA3, ubiquitin-activating enzyme E1 homolog,ubiquitin-activating enzyme 3,ubiquitin-activating enzyme E1C (UBA3 homolog, yeast),ubiquitin-activating enzyme E1C (homologous to yeast UBA3)
- Gene Pathway